Pramod is a Call 3 fellow and his project is on cell based therapy for respiratory epithelium tissue regeneration. Pramod completed his PhD in Biomedical Engineering at NUI Galway, Ireland. Before moving to Ireland he completed a M. Pharm. from Dr H S Gour University, Sagar India. His research interests are in vitro tissue regeneration, development of biomaterials, drug delivery and nanotechnology.
Brief description of research project
The regeneration of functional ciliated respiratory epithelium is an utmost surgical and scientific need for the treatment of several life threatening respiratory diseases. Pramod’s project is on in vitro development of organised respiratory epithelium using the growth factor delivery. In this project, he is developing the extracellular matrix based hydrogel for the controlled delivery of growth factors to stimulate the epithelium regeneration in vitro.
The native extracellular matrix based films (gelatin and gelatin-HA) have been fabricated using the spin coating method and loaded with the potent epithelium cell specific growth factors (FGF-7 and FGF-10). Bone marrow derived mesenchymal cells (MSCs) have been cultured on these gelatin based films and exposed to the sustained released growth factors from film to induce the epithelium differentiation. Following three-four weeks of submerged culture the system was exposed to the air-liquid interface culture conditions. It was observed that the GFs loaded gelatin and gelatin-HA films facilitate the epithelium differentiation of MSCs. The cells start the expression of various epithelium markers, e.g. Pan cytokeratin, CK-18 as well as wide spectrum cytokeratin and reduce the expression of MSCs marker vimentin. Also, the stem cells spindle shaped morphology changes to the cobblestone shaped epithelium morphology within two week in-vitro culture. However, the system was unable to induce the respiratory epithelium characteristics in MSCs derived epithelium due to lack of some important signals. Further optimisation of in-vitro microenvironment is underway to optimise such complex tissue development.